HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Separate, identify and quantify substances
Choose an experiment, set a simple HPLC method, run standards and samples, then interpret Rt and peak area.
Mobile phase→Pump→Injector→Column→Detector
Rt: time from injection to detection.
Peak area: proportional to analyte amount under controlled conditions.
Important: compare standards and samples only when all HPLC conditions match.
INSTRUMENT FLOW PATH
Configure the HPLC method
Ready
Mobile phase
Water : Ethanol
Chosen automatically from the stationary phase
Polarity
Non-polar
Hover or tap i for component information
Ratio60% : 40%
ColumnNon-polar · 100 mm
Flow1.00 mL/min
VialNo vial selected
Starting method — poor separation expected.
Varying conditions during experiments will invalidate your results.
Pumping mobile phase…
DETECTOR OUTPUT
Chromatogram
Fixed axes: 0–20 min · common response scale
Run a standard or sample to produce a chromatogram.
RESULTS AND CONCLUSION